A fine-tuned yeast surface-display/secretion platform enables the rapid discovery of neutralizing antibodies against Clostridioides difficile toxins.

ABSTRACT:

Background

Neutralizing antibody plays a key role in protecting hosts from invasive pathogens and their virulent components. Current high-throughput assays for antibody screening are based on binding activities. However, those antibodies with high affinity may not have neutralizing activities. Subsequent functionality assays are necessary to identify neutralizing antibodies from binders with high affinity to their target antigens, which is laborious and time-consuming. Therefore, a versatile platform that can rapidly identify antibodies with both high binding affinity and neutralizing activity is desired to curb future pandemics like COVID-19.

Results

In this proof-of-concept study, we adapted Saccharomyces cerevisiae to either display human antibodies on the yeast surface o

SUBMITTER: Sun Y 

PROVIDER: S-EPMC10519002 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature