Septins regulate border cell surface geometry, shape, and motility downstream of Rho in Drosophila.

ABSTRACT: Septins self-assemble into polymers that bind and deform membranes in vitro and regulate diverse cell behaviors in vivo. How their in vitro properties relate to their in vivo functions is under active investigation. Here, we uncover requirements for septins in detachment and motility of border cell clusters in the Drosophila ovary. Septins and myosin colocalize dynamically at the cluster periphery and share phenotypes but, surprisingly, do not impact each other. Instead, Rho independently regulates myosin activity and septin localization. Active Rho recruits septins to membranes, whereas inactive Rho sequesters septins in the cytoplasm. Mathematical analyses identify how manipulating septin expression levels alters cluster surface texture and shape. This study shows that the level of septi

SUBMITTER: Gabbert AM 

PROVIDER: S-EPMC10519140 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature