Structure and Dynamics of DNA and RNA Double Helices Formed by d(CTG), d(GTC), r(CUG), and r(GUC) Trinucleotide Repeats and Associated DNA-RNA Hybrids.

ABSTRACT: Myotonic dystrophy type 1 is the most frequent form of muscular dystrophy in adults caused by an abnormal expansion of the CTG trinucleotide. Both the expanded DNA and the expanded CUG RNA transcript can fold into hairpins. Co-transcriptional formation of stable RNA·DNA hybrids can also enhance the instability of repeat tracts. We performed molecular dynamics simulations of homoduplexes associated with the disease, d(CTG)_n and r(CUG)_n, and their corresponding r(CAG)_n:d(CTG)_n and r(CUG)_n:d(CAG)_n hybrids that can form under bidirectional transcription and of non-pathological d(GTC)_n and d(GUC)_n homoduplexes. We characterized their conformations, stab

SUBMITTER: Fakharzadeh A 

PROVIDER: S-EPMC10519205 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature