Genetic control of the dynamic transcriptional response to immune stimuli and glucocorticoids at single-cell resolution.

ABSTRACT: Synthetic glucocorticoids, such as dexamethasone, have been used as a treatment for many immune conditions, such as asthma and, more recently, severe COVID-19. Single-cell data can capture more fine-grained details on transcriptional variability and dynamics to gain a better understanding of the molecular underpinnings of inter-individual variation in drug response. Here, we used single-cell RNA-seq to study the dynamics of the transcriptional response to glucocorticoids in activated peripheral blood mononuclear cells from 96 African American children. We used novel statistical approaches to calculate a mean-independent measure of gene expression variability and a measure of transcriptional response pseudotime. Using these approaches, we showed that glucocorticoids reverse the effects of i

SUBMITTER: Resztak JA 

PROVIDER: S-EPMC10519413 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature