MYC-MAX heterodimerization is essential for the induction of major zygotic genome activation and subsequent preimplantation development.

ABSTRACT: In mouse preimplantation development, zygotic genome activation (ZGA), which synthesizes new transcripts in the embryo, begins in the S phase at the one-cell stage, with major ZGA occurring especially at the late two-cell stage. Myc is a transcription factor expressed in parallel with ZGA, but its direct association with major ZGA has not been clarified. In this study, we found that developmental arrest occurs at the two-cell stage when mouse embryos were treated with antisense oligonucleotides targeting Myc or MYC-specific inhibitors from the one-cell stage. To identify when MYC inhibition affects development, we applied time-limited inhibitor treatment and found that inhibition of MYC at the one-cell, four-cell, and morula stages had no effect on preimplantation development, whereas inhi

SUBMITTER: Yamamoto T 

PROVIDER: S-EPMC10520005 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature