Construction of IgG-Fab² bispecific antibody via intein-mediated protein trans-splicing reaction.

ABSTRACT: A bispecific antibody (bsAb) is a class of engineered antibody molecules that simultaneously binds to two different antigens by having two kinds of antigen-binding domains. One of the major obstacles for the bsAb production is the incorrect chain-pairing problem, wherein each heavy and light chain should form pairings with the correct counterpart's chains, but the structural similarity of the incorrect partners also forms the incorrect pairings. This study aimed to demonstrate a bsAb construction method using intein-mediated protein trans-splicing to create IgG-Fab²-type bsAbs, which is a modified antibody with a structure in which two additional Fabs are linked to the N-terminus of the heavy chain of an IgG molecule. The chain-paring problem between a heavy chain and a light ch

SUBMITTER: Yamada R 

PROVIDER: S-EPMC10520027 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature