An Improved Model for Circular RNA Overexpression: Using the Actin Intron Reveals High Circularization Efficiency.

ABSTRACT: Traditionally, the group 1 intron of the T4 td gene is used to generate a foreign circular sequence. However, the T4 system has been shown to be fairly inefficient in expressing circular RNA (circRNA). Here, a new method is developed to express circular sequences with high circularization efficiency to strengthen the confidence for future circRNA functional studies. CircRNA expression plasmids, constructed with different lengths derived from the actin intron (15-nt, 30-nt, 60-nt, 100-nt, 180-nt) and T4 intron, are introduced into human and mouse cell lines 293T and B16. Junction detection and sequencing are used to determine successful circularization of introns and their expression efficiencies. An actin intron with a medium length (60-nt-100-nt) shows significantly increased effic

SUBMITTER: Li F 

PROVIDER: S-EPMC10520416 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature