Global quantitative understanding of non-equilibrium cell fate decision-making in response to pheromone.

ABSTRACT: Cell-cycle arrest and polarized growth are commonly used to characterize the response of yeast to pheromone. However, the quantitative decision-making processes underlying time-dependent changes in cell fate remain unclear. In this study, we conducted single-cell level experiments to observe multidimensional responses, uncovering diverse fates of yeast cells. Multiple states are revealed, along with the kinetic switching rates and pathways among them, giving rise to a quantitative landscape of mating response. To quantify the experimentally observed cell fates, we developed a theoretical framework based on non-equilibrium landscape and flux theory. Additionally, we performed stochastic simulations of biochemical reactions to elucidate signal transduction and cell growth. Notably, our exper

SUBMITTER: Li S 

PROVIDER: S-EPMC10520453 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature