NLRP14 Safeguards Calcium Homeostasis via Regulating the K27 Ubiquitination of Nclx in Oocyte-to-Embryo Transition.

ABSTRACT: Sperm-induced Ca²⁺ rise is critical for driving oocyte activation and subsequent embryonic development, but little is known about how lasting Ca²⁺ oscillations are regulated. Here it is shown that NLRP14, a maternal effect factor, is essential for keeping Ca²⁺ oscillations and early embryonic development. Few embryos lacking maternal NLRP14 can develop beyond the 2-cell stage. The impaired developmental potential of Nlrp14-deficient oocytes is mainly caused by disrupted cytoplasmic function and calcium homeostasis due to altered mitochondrial distribution, morphology, and activity since the calcium oscillations and development of Nlrp14-deficient oocytes can be rescued by substitution of whole cytoplasm by spindle transfer. Proteomics analysis reveal that c

SUBMITTER: Meng TG 

PROVIDER: S-EPMC10520637 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature