The location and development of Replicon Cluster Domains in early replicating DNA.

ABSTRACT: Background: It has been known for many years that in metazoan cells, replication origins are organised into clusters where origins within each cluster fire near-synchronously. Despite clusters being a fundamental organising principle of metazoan DNA replication, the genomic location of origin clusters has not been documented. Methods: We synchronised human U2OS by thymidine block and release followed by L-mimosine block and release to create a population of cells progressing into S phase with a high degree of synchrony. At different times after release into S phase, cells were pulsed with EdU; the EdU-labelled DNA was then pulled down, sequenced and mapped onto the human genome. Results: The early replicating DNA showed features at a range of scales. Wavelet analysis s

SUBMITTER: da Costa-Nunes JA 

PROVIDER: S-EPMC10521077 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature