Molecular characterization of gliotoxin-producing Aspergillus fumigatus in dairy cattle feed.

ABSTRACT:

Background and aim

Several strains of Aspergillus fumigatus produce mycotoxins that affect the health and productivity of dairy cattle, and their presence in dairy cattle feed is a serious concern. This study aimed to determine the densities of A. fumigatus and gliotoxin in commercial dairy feed.

Materials and methods

More than 60 dairy feed samples were examined for fungal contamination, specifically for A. fumigatus, using phenotypic approaches and DNA sequencing of the internal transcribed spacer (ITS) and β-tubulin regions. Thin-layer chromatography and high-performance liquid chromatography (HPLC) were used to assess gliotoxin production in A. fumigatus. Real-time polymerase chain reaction (RT-PCR) was used to investigate the ex

SUBMITTER: Mohamed HMA 

PROVIDER: S-EPMC10521192 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature