Nano-seq analysis reveals different functional tendency between exosomes and microvesicles derived from hUMSC.

ABSTRACT:

Background

Extracellular vesicles (EVs) from human umbilical cord mesenchymal stem cells (hUMSCs) are widely considered to be the best mediators for cell-free therapy. An understanding of their composition, especially RNA, is particularly important for the safe and precise application of EVs. Up to date, the knowledge of their RNA components is limited to NGS sequencing and cannot provide a comprehensive transcriptomic landscape, especially the long and full-length transcripts. Our study first focused on the transcriptomic profile of hUMSC-EVs based on nanopore sequencing.

Methods

In this study, different EV subtypes (exosomes and microvesicles) derived from hUMSCs were isolated and identified by density gradient centrifugation. Subsequently, the realistic long transcriptomi

SUBMITTER: Yu D 

PROVIDER: S-EPMC10521478 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature