Protocol: an improved method for inducing sporophyte generation in the model moss Physcomitrium patens under nitrogen starvation.

ABSTRACT:

Background

Land plants exhibit a haplodiplontic life cycle, whereby multicellular bodies develop in both the haploid and diploid generations. The early-diverging land plants, known as bryophytes, have a haploid-dominant life cycle, in which a short-lived multicellular body in the diploid generation, known as the sporophyte, develops on the maternal haploid gametophyte tissues. The moss Physcomitrium (Physcomitrella) patens has become one of the most powerful model systems in evolutionary plant developmental studies. To induce diploid sporophytes of P. patens, several protocols are implemented. One of the conventional approaches is to grow approximately one-month-old gametophores for another month on Jiffy-7 pellets made from the peat moss that is difficult to fully sterilize. A mor

SUBMITTER: Yoro E 

PROVIDER: S-EPMC10521525 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature