BAX activation in mouse retinal ganglion cells occurs in two temporally and mechanistically distinct steps.

ABSTRACT:

Background

Pro-apoptotic BAX is a central mediator of retinal ganglion cell (RGC) death after optic nerve damage. BAX activation occurs in two stages including translocation of latent BAX to the mitochondrial outer membrane (MOM) and then permeabilization of the MOM to facilitate the release of apoptotic signaling molecules. As a critical component of RGC death, BAX is an attractive target for neuroprotective therapies and an understanding of the kinetics of BAX activation and the mechanisms controlling the two stages of this process in RGCs is potentially valuable in informing the development of a neuroprotective strategy.

Methods

The kinetics of BAX translocation were assessed by both static and live-cell imaging of a GFP-BAX fusion protein introduced into RGCs using AAV2-

SUBMITTER: Maes ME 

PROVIDER: S-EPMC10521527 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature