Function and autophagy of monocyte-derived dendritic cells is affected by hepatitis B virus infection.

ABSTRACT:

Background

The role of dendritic cells and the autophagy state of dendritic cells in the immune response of hepatitis B virus (HBV) infection was still controversial. In this study, we carefully examined the phenotype, function and autophagy pathway of dendritic cells in HBV infection.

Methods

Monocyte-derived dendritic cells from healthy blood donors and patients with chronic HBV infection were stimulated by lipopolysaccharide, supernatant of HepG2.2.15 cells or supernatant of HepG2 cells respectively. Phenotype of dendritic cells was examined by flow cytometry and cytokines secretion was detected by enzyme-linked immunosorbent assay. Autophagy related proteins were detected by western blot and immunofluorescence analysis.

Results

Our results showed that the expressi

SUBMITTER: Xu H 

PROVIDER: S-EPMC10521579 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature