Engineering a Circular Riboregulator in Escherichia coli.
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ABSTRACT:
SUBMITTER: Rostain W
PROVIDER: S-EPMC10521646 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
Engineering a Circular Riboregulator in <i>Escherichia coli</i>.
Rostain William W Shen Shensi S Cordero Teresa T Rodrigo Guillermo G Jaramillo Alfonso A
Biodesign research 20200912
RNAs of different shapes and sizes, natural or synthetic, can regulate gene expression in prokaryotes and eukaryotes. Circular RNAs have recently appeared to be more widespread than previously thought, but their role in prokaryotes remains elusive. Here, by inserting a riboregulatory sequence within a group I permuted intron-exon ribozyme, we created a small noncoding RNA that self-splices to produce a circular riboregulator in <i>Escherichia coli</i>. We showed that the resulting riboregulator ...[more]
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