In-Depth Computational Analysis of Natural and Artificial Carbon Fixation Pathways.

ABSTRACT: In the recent years, engineering new-to-nature CO₂- and C1-fixing metabolic pathways made a leap forward. New, artificial pathways promise higher yields and activity than natural ones like the Calvin-Benson-Bassham (CBB) cycle. The question remains how to best predict their in vivo performance and what actually makes one pathway "better" than another. In this context, we explore aerobic carbon fixation pathways by a computational approach and compare them based on their specific activity and yield on methanol, formate, and CO₂/H₂ considering the kinetics and thermodynamics of the reactions. Besides pathways found in nature or implemented in the laboratory, this included two completely new cycles with favorable features: the reductive citramalyl-CoA c

SUBMITTER: Lowe H 

PROVIDER: S-EPMC10521678 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature