Adaptive Design of Fluorescence Imaging Systems for Custom Resolution, Fields of View, and Geometries.

ABSTRACT: Objective and Impact Statement: We developed a generalized computational approach to design uniform, high-intensity excitation light for low-cost, quantitative fluorescence imaging of in vitro, ex vivo, and in vivo samples with a single device. Introduction: Fluorescence imaging is a ubiquitous tool for biomedical applications. Researchers extensively modify existing systems for tissue imaging, increasing the time and effort needed for translational research and thick tissue imaging. These modifications are application-specific, requiring new designs to scale across sample types. Methods: We implemented a computational model to simulate light propagation from multiple sources. Using a global optimization algorithm and a custom cost function, we determined the spatial p

SUBMITTER: Wang R 

PROVIDER: S-EPMC10521686 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature