Benchmarking Intrinsic Promoters and Terminators for Plant Synthetic Biology Research.

ABSTRACT: The emerging plant synthetic metabolic engineering has been exhibiting great promise to produce either value-added metabolites or therapeutic proteins. However, promoters for plant pathway engineering are generally selected empirically. The quantitative characterization of plant-based promoters is essential for optimal control of gene expression in plant chassis. Here, we used N. benthamiana leaves and BY2 suspension cells to quantitatively characterize a library of plant promoters by transient expression of firefly/Renilla luciferase. We validated the dual-luciferase reporter system by examining the correlation between reporter protein and mRNA levels. In addition, we investigated the effects of terminator-promoter combinations on gene expression and found that the combinati

SUBMITTER: Tian C 

PROVIDER: S-EPMC10521690 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature