Altered Carbon Partitioning Enhances CO₂ to Terpene Conversion in Cyanobacteria.

ABSTRACT: Photosynthetic terpene production represents one of the most carbon and energy-efficient routes for converting CO₂ into hydrocarbon. In photosynthetic organisms, metabolic engineering has led to limited success in enhancing terpene productivity, partially due to the low carbon partitioning. In this study, we employed systems biology analysis to reveal the strong competition for carbon substrates between primary metabolism (e.g., sucrose, glycogen, and protein synthesis) and terpene biosynthesis in Synechococcus elongatus PCC 7942. We then engineered key "source" and "sink" enzymes. The "source" limitation was overcome by knocking out either sucrose or glycogen biosynthesis to significantly enhance limonene production via altered carbon partitioning. Moreover, a fus

SUBMITTER: Li M 

PROVIDER: S-EPMC10521692 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature