Revealing CO₂-Fixing SAR11 Bacteria in the Ocean by Raman-Based Single-Cell Metabolic Profiling and Genomics.

ABSTRACT: The majority of marine microbes remain uncultured, which hinders the identification and mining of CO₂-fixing genes, pathways, and chassis from the oceans. Here, we investigated CO₂-fixing microbes in seawater from the euphotic zone of the Yellow Sea of China by detecting and tracking their ¹³C-bicarbonate (¹³C-HCO₃^-) intake via single-cell Raman spectra (SCRS) analysis. The target cells were then isolated by Raman-activated Gravity-driven Encapsulation (RAGE), and their genomes were amplified and sequenced at one-cell resolution. The single-cell metabolism, phenotype and genome are consistent. We identified a not-yet-cultured Pelagibacter spp., which actively assimilates ¹³C-HCO₃^-, and

SUBMITTER: Jing X 

PROVIDER: S-EPMC10521720 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature