Endothelial Cell APOE3 Regulates Neurovascular, Neuronal, and Behavioral Function.

ABSTRACT:

Background

Specialized brain endothelial cells and human APOE3 are independently important for neurovascular function, yet whether APOE3 expression by endothelial cells contributes to brain function is currently unknown. In the present study, we determined whether the loss of endothelial cell APOE3 impacts brain vascular and neural function.

Methods

We developed APOE3^fl/fl/Cdh5(PAC)-CreERT2^+/- (APOE3^Cre+/-) and APOE3^fl/fl/Cdh5(PAC)-CreERT2^-/- (APOE3^Cre-/-, control) mice and induced endothelial cell APOE3 knockdown with tamoxifen at ≈4 to 5 weeks of age. Neurovascular and neuronal function were evaluated by biochemistry, immunohistochemistry, be

SUBMITTER: Marottoli FM 

PROVIDER: S-EPMC10521805 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature