TIMP1 shapes an immunosuppressive microenvironment by regulating anoikis to promote the progression of clear cell renal cell carcinoma.

ABSTRACT:

Background

The association between ccRCC and Anoikis remains to be thoroughly investigated.

Methods

Anoikis-related clusters were identified using NMF. To identify prognostic anoikis-related genes (ARGs) and establish an optimal prognostic model, univariate Cox and LASSO regression were employed. The E-MTAB-1980 cohort was utilized for external validation. Multiple algorithms were used to evaluate the immune properties of the model. GO, KEGG and GSVA analyses were employed to analyze biological pathway functions. qRT-PCR was employed to measure RNA levels of specific genes. Cell Counting Kit-8, wound healing, and Transwell chamber assays were performed to determine changes in the proliferative and metastatic abilities of A498 and 786-O cells.

Results

Based on the expr

SUBMITTER: Li Q 

PROVIDER: S-EPMC10522382 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature