Polydimethylsiloxane as a more biocompatible alternative to glass in optogenetics.

ABSTRACT: Optogenetics is highly useful to stimulate or inhibit defined neuronal populations and is often used together with electrophysiological recordings. Due to poor penetration of light in tissue, there is a need for biocompatible wave guides. Glass wave guides are relatively stiff and known to cause glia reaction that likely influence the activity in the remaining neurons. We developed highly flexible micro wave guides for optogenetics that can be used in combination with long-lasting electrophysiological recordings. We designed and evaluated polydimethylsiloxane (PDMS) mono-fibers, which use the tissue as cladding, with a diameter of 71 ± 10 µm and 126 ± 5 µm. We showed that micro PDMS fibers transmitted 9-33 mW/mm² light energy enough to activate channelrhodopsin. This was confirm

SUBMITTER: Andersen MA 

PROVIDER: S-EPMC10522705 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature