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Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context.

ABSTRACT: Recent technical advances, such as single-cell RNA sequencing and mass cytometry, improve identification of cell types and subsets in a range of healthy and diseased tissues at the expense of their cellular and molecular context. Here, we present a protocol for in situ multispectral imaging to map myeloid cell heterogeneity in tissue cryosections, describing steps for cutting sequential sections, antibody titration, and building a spectral library. We then detail procedures for multispectral imaging and preparing data for downstream analysis. For complete details on the use and execution of this protocol, please refer to Goossens et al. (2022).¹.

SUBMITTER: Wieland EB

PROVIDER: S-EPMC10522972 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context.

Wieland Elias B EB Kempen Laura J A P LJAP Lu Chang C Donners Marjo M P C MMPC Biessen Erik A L EAL Goossens Pieter P

STAR protocols 20230923 4

Recent technical advances, such as single-cell RNA sequencing and mass cytometry, improve identification of cell types and subsets in a range of healthy and diseased tissues at the expense of their cellular and molecular context. Here, we present a protocol for in situ multispectral imaging to map myeloid cell heterogeneity in tissue cryosections, describing steps for cutting sequential sections, antibody titration, and building a spectral library. We then detail procedures for multispectral ima ...[more]

PMID: 37742177

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Dataset Information

Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context.

Publications

Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context.

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