CREB-Regulated Transcriptional Coactivator 2 Proteome Landscape is Modulated by SREBF1.

ABSTRACT: cAMP response element-binding protein (CREB) regulated transcriptional coactivator 2 (CRTC2) is a critical transcription factor that maintains glucose homeostasis by activating CREB. Energy homeostasis is maintained through multiple pathways; therefore, CRTC2 may interact with other transcription factors, particularly under metabolic stress. CRTC2 liver-specific KO mice were created, and the global proteome, phosphoproteome, and acetylome from liver tissue under high-fat diet conditions were analyzed using liquid chromatography-tandem mass spectrometry and bioinformatics analysis. Differentially regulated proteins (DRPs) were enriched in metabolic pathways, which were subsequently corroborated through animal experiments. The consensus DRPs from these datasets were used as seed proteins to

SUBMITTER: Lim JM 

PROVIDER: S-EPMC10522995 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature