The central nervous system is a potential reservoir and possible origin of drug resistance in hepatitis B infection.

ABSTRACT:

Background

The significance of hepatitis B virus (HBV) in cerebrospinal fluid (CSF) is unclear.

Methods

Synchronous serum and CSF samples were collected from 13 patients. HBV DNA, full-length genome, quasispecies, phylogenetic tree, compartmentalization and mutation of the reverse transcriptase (RT) region were performed based on PCR and sequencing methods.

Results

HBV DNA was detected in the CSF of 3 antiviral-naïve individuals and 1 individual after successful antiviral therapy. Complete full-length HBV genomes were isolated from the CSF of 5 individuals, including 2 with undetectable serum HBV DNA. Ten individuals exhibited distinct CSF-serum quasispecies, 8 harbored independent CSF-serum genetic compartmentalization and phylogenetic trees, and 5 lamivudine/entecav

SUBMITTER: Xu L 

PROVIDER: S-EPMC10523273 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature