P38^MAPK/MK2 signaling stimulates host cells autophagy pathways to restrict Salmonella infection.

ABSTRACT: Autophagy plays an important role in recognizing and protecting cells from invading intracellular pathogens such as Salmonella. In this work, we investigated the role of p38^MAPK/MK2 in modulating the host cell susceptibility to Salmonella infection. Inhibition of p38^MAPK or MK2 led to a significant increase of bacterial counts in Salmonella infected mouse embryonic fibroblasts (MEFs), as well as in MK2-deficient (Mk2^-/-) cells. Furthermore, western blot analysis showed that Mk2^-/- cells have lower level of LC3 lipidation, which is the indicator of general autophagy compared to Mk2-rescued cells. In Mk2^-/- cells, we also observed lower activated TANK-binding kinase-1 phosphorylation on Se

SUBMITTER: Suwandi A 

PROVIDER: S-EPMC10523304 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature