Dynamic cytological and transcriptomic analyses provide novel insights into the mechanisms of sex determination in Castanea henryi.

ABSTRACT: Castanea henryi is a monoecious woody food tree species whose yield and industrialization potential are limited by its low female-to-male flower ratio. Here, the male flowers on the male inflorescence of C. henryi were converted to female flowers by triple applications of exogenous cytokinin (CK) (N-(2-chloro-4-pyridyl)-N'-phenylurea, CPPU). To study the role of exogenous CK in flower sex determination, cytological and transcriptomic analyses were performed on samples from the five stages after CK treatment. Cytological analysis showed that stage 3 (nine days after the last CK treatment) was the critical stage in the differential development of the pistil primordium and stamen primordium. On this basis, one key module and two modules with significant positive correlations wit

SUBMITTER: Wu G 

PROVIDER: S-EPMC10523332 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature