ATP Acts as a Hydrotrope to Regulate the Phase Separation of NBDY Clusters.

ABSTRACT: Nonannotated P-body dissociating polypeptide (NBDY) is a recently discovered human microprotein that has been found to be a novel component of the mRNA decapping complex. Previous studies have shown that the phosphorylation of NBDY promotes the liquid phase of the NBDY remixing in vitro. Typically, during the process of phosphorylation, a phosphate group is added to the protein through adenosine triphosphate (ATP) hydrolysis. It has been shown that ATP acts as a biological hydrotrope, affecting the phase separation of proteins in solution. In this study, we utilized simulation methods to investigate the dynamic properties of the NBDY clusters at various ATP concentrations. Our findings demonstrate that ATP can regulate the phase separation of NBDY clusters. Specifically, we identified a cr

SUBMITTER: Liu F 

PROVIDER: S-EPMC10523361 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature