Phenotypic features of genetically modified DMD-X^KOX^WT pigs.

ABSTRACT:

Introduction

Duchenne muscular dystrophy (DMD) is a hereditary neuromuscular disorder caused by mutation in the dystrophin gene (DMD) on the X chromosome. Female DMD carriers occasionally exhibit symptoms such as muscle weakness and heart failure. Here, we investigated the characteristics and representativeness of female DMD carrier (DMD-X^KOX^WT) pigs as a suitable disease model.

Methods

In vitro fertilization using sperm from a DMD-X^KOY↔X^WTX^WT chimeric boar yielded DMD-X^KOX^WT females, which were used to generate F2 and F3 progeny, including DMD-X^KOX^WT females. F1-F3 piglets were genotyped and subjected to biochemical analysis for blood creatin

SUBMITTER: Okamoto K 

PROVIDER: S-EPMC10523442 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature