Transcriptional characteristics and functional validation of three monocyte subsets during aging.

ABSTRACT:

Background

Age-associated changes in immunity are inextricably linked to chronic inflammation and age-related diseases, the impact of aging on monocyte subsets is poorly understood.

Methods

Flow cytometry was applied to distinguish three monocyte subsets between 120 young and 103 aged individuals. We then analyzed the expression profiles of three monocyte subsets from 9 young and 9 older donors and CD14⁺ monocytes from 1202 individuals between 44 and 83 years old. Flow cytometry was used to measure β-galactosidase activities, ROS levels, mitochondrial contents, mitochondrial membrane potentials (MMPs) and intracellular IL-6 levels in three monocyte subsets of young and elderly individuals, and plasma IL-6 levels were detected by electrochemiluminescence immunoassa

SUBMITTER: Wang C 

PROVIDER: S-EPMC10523626 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature