Collagen/kerateine multi-protein hydrogels as a thermally stable extracellular matrix for 3D in vitro models.

ABSTRACT: Objective: To determine whether the addition of kerateine (reduced keratin) in rat tail collagen type I hydrogels increases thermal stability and changes material properties and supports cell growth for use in cellular hyperthermia studies for tumor treatment.Methods: Collagen type I extracted from rat tail tendon was combined with kerateine extracted from human hair fibers. Thermal, mechanical, and biocompatibility properties and cell behavior was assessed and compared to 100% collagen type I hydrogels to demonstrate their utility as a tissue model for 3D in vitro testing.Results: A combination (i.e., containing both collagen 'C/KNT') hydrogel was more thermally stable than pure collagen hydrogels and resisted thermal degradation when incubated at a hyperthermi

SUBMITTER: Zuniga K 

PROVIDER: S-EPMC10523628 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature