Towards a better preclinical cancer model - human immune aging in humanized mice.

ABSTRACT:

Background

Preclinical models are often used for cancer studies and evaluation of novel therapeutics. The relevance of these models has vastly improved with mice bearing a human immune system, especially in the context of immunotherapy. Nonetheless, cancer is an age-related disease, and studies often overlook the effects of aging. Here we have established a humanized mouse model of human immune aging to investigate the role of this phenomenon on liver tumor dynamics.

Methods

Multiple organs and tissues (blood, thymus, lung, liver, spleen and bone marrow) were harvested from NOD-scid IL2rγ^-/- (NIKO) mice reconstituted with human immune cells, over a period of 60 weeks post-birth, for immune profiling. Young and aging immune cells were compared for transcriptomic ch

SUBMITTER: Tan JHL 

PROVIDER: S-EPMC10523735 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature