ScRNA-seq revealed disruption in CD8⁺ NKG2A⁺ natural killer T cells in patients after liver transplantation and immunosuppressive therapy.

ABSTRACT:

Background

Liver transplantation (LT) offers a good survival chance for both the patient in short or long term, but still faces many challenges in the treatment of LT, such as the side effects associated with long-term immunosuppression, which is one of the side effects that occurs in most patients. However, the dynamics of the cellular immune system composition over time during immune tolerance to LT after immunosuppressive therapy are not known.

Methods

Using single-cell transcriptome sequencing, we analyzed five peripheral blood samples (one normal individual and four patients who underwent LT and received immunosuppressive therapy for 2 months, 1 year, 3 years, and 7 years, respectively) for immune cell composition and gene expression.

Results

A total of 17,462 pe

SUBMITTER: Fang Y 

PROVIDER: S-EPMC10524014 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature