In vivo screening identifies SPP2, a secreted factor that negatively regulates liver regeneration.

ABSTRACT:

Background and aims

The liver is remarkably regenerative and can completely recover even when 80% of its mass is surgically removed. Identification of secreted factors that regulate liver growth would help us understand how organ size and regeneration are controlled but also provide candidate targets to promote regeneration or impair cancer growth.

Approach and results

To enrich for secreted factors that regulate growth control, we induced massive liver overgrowth with either YAP or MYC . Differentially expressed secreted factors were identified in these livers using transcriptomic analysis. To rank candidates by functionality, we performed in vivo CRISPR screening using the Fah knockout model of tyrosinemia. We identified secreted phosphoprotein-2 (SPP2) as a secreted facto

SUBMITTER: Lin YH 

PROVIDER: S-EPMC10524179 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature