Age and oxidative stress regulate Nrf2 homeostasis in human articular chondrocytes.

ABSTRACT:

Objective

The purpose of this study was to investigate the effect of age and oxidative stress on regulation of nuclear factor erythroid-2-related factor 2 (Nrf2) in young, old, and osteoarthritic (OA) human articular chondrocytes.

Design

Levels of Nrf2 in primary human chondrocytes isolated from young, old, and OA donors were measured by immunoblotting, qPCR, and immunohistochemistry. Effects on levels of Nrf2, antioxidant proteins regulated by Nrf2, as well as p65, and the anabolic response to insulin-like growth factor-1 (IGF-1) were evaluated after induction of oxidative stress with menadione, Nrf2 knockdown with siRNA, and/or Nrf2 activation with RTA-408.

Results

Nrf2 protein levels were significantly lower in older adult chondrocytes (∼0.59 fold; p = 0.034) and O

SUBMITTER: Taylor EL 

PROVIDER: S-EPMC10524306 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature