Single-cell transcriptomics reveal a hyperacute cytokine and immune checkpoint axis after cardiac arrest in patients with poor neurological outcome.

ABSTRACT:

Background

Most patients hospitalized after cardiac arrest (CA) die because of neurological injury. The systemic inflammatory response after CA is associated with neurological injury and mortality but remains poorly defined.

Methods

We determine the innate immune network induced by clinical CA at single-cell resolution.

Findings

Immune cell states diverge as early as 6 h post-CA between patients with good or poor neurological outcomes 30 days after CA. Nectin-2⁺ monocyte and Tim-3⁺ natural killer (NK) cell subpopulations are associated with poor outcomes, and interactome analysis highlights their crosstalk via cytokines and immune checkpoints. Ex vivo studies of peripheral blood cells from CA patients demonstrate that immune checkpoints are a com

SUBMITTER: Tamura T 

PROVIDER: S-EPMC10524451 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature