Siglec-9 is an inhibitory receptor on human mast cells in vitro.

ABSTRACT:

Background

Mast cell activation is critical for the development of allergic diseases. Ligation of sialic acid-binding immunoglobin-like lectins (Siglecs), such as Siglec-6, -7, and -8 as well as CD33, have been shown to inhibit mast cell activation. Recent studies showed that human mast cells express Siglec-9, an inhibitory receptor also expressed by neutrophils, monocytes, macrophages, and dendritic cells.

Objective

We aimed to characterize Siglec-9 expression and function in human mast cells in vitro.

Methods

We assessed the expression of Siglec-9 and Siglec-9 ligands on human mast cell lines and human primary mast cells by real-time quantitative PCR, flow cytometry, and confocal microscopy. We used a clustered regularly interspaced short palindromic repeats (CRISPR

SUBMITTER: Miralda I 

PROVIDER: S-EPMC10524464 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature