Subsets of IFN Signaling Predict Response to Immune Checkpoint Blockade in Patients with Melanoma.

ABSTRACT:

Purpose

IFN signaling in the tumor microenvironment is a critical determinant of both response and resistance of cancer to immune checkpoint inhibitors (ICI). We hypothesized that distinct patterns of IFN signaling in melanoma are associated with clinical response or resistance to ICIs.

Experimental design

Two tissue microarrays containing samples from 97 patients with metastatic melanoma who received nivolumab, pembrolizumab, or a combination of ipilimumab and nivolumab at Yale New Haven Hospital between 2011 and 2017 were randomized into discovery and validation cohorts. Samples were stained and visualized using multiplexed immunofluorescence microscopy for STAT1, STAT1 phosphorylated at Y701 (pSTAT1Y701), and PD-L1, and signals were quantified using the automated quantita

SUBMITTER: Horowitch B 

PROVIDER: S-EPMC10524955 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature