Transcriptome Studies Reveal the N⁶-Methyladenosine Differences in Testis of Yaks at Juvenile and Sexual Maturity Stages.

ABSTRACT: Studying the mechanism of spermatogenesis is key to exploring the reproductive characteristics of male yaks. Although N⁶-methyladenosine (m⁶A) RNA modification has been reported to regulate spermatogenesis and reproductive function in mammals, the molecular mechanism of m⁶A in yak testis development and spermatogenesis remains largely unknown. Therefore, we collected testicular tissue from juvenile and adult yaks and found that the m⁶A level significantly increased after sexual maturity in yaks. In MeRIP-seq, 1702 hypermethylated peaks and 724 hypomethylated peaks were identified. The hypermethylated differentially methylated RNAs (DMRs) (CIB2, AK1, FOXJ2, PKDREJ, SLC9A3, and TOPAZ1) mainly regulated spermatogenesis. Functional enrichment

SUBMITTER: Guo S 

PROVIDER: S-EPMC10525320 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature