Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress.

ABSTRACT: Bacterial ribosome small subunit rRNA (16S rRNA) contains 11 nucleotide modifications scattered throughout all its domains. The 16S rRNA pseudouridylation enzyme, RsuA, which modifies U516, is a survival protein essential for bacterial survival under stress conditions. A comparison of the growth curves of wildtype and RsuA knock-out E. coli strains illustrates that RsuA renders a survival advantage to bacteria under streptomycin stress. The RsuA-dependent growth advantage for bacteria was found to be dependent on its pseudouridylation activity. In addition, the role of RsuA as a trans-acting factor during ribosome biogenesis may also play a role in bacterial growth under streptomycin stress. Furthermore, circular dichroism spectroscopy measurements and RNase footprinting studies hav

SUBMITTER: Abedeera SM 

PROVIDER: S-EPMC10525438 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature