A Reappraisal of the Utility of L-012 to Measure Superoxide from Biologically Relevant Sources.

ABSTRACT: The detection of superoxide anion (O₂^●-) in biological tissues remains challenging. Barriers to convenient and reproducible measurements include expensive equipment, custom probes, and the need for high sensitivity and specificity. The luminol derivative, L-012, has been used to measure O₂^●- since 1993 with mixed results and concerns over specificity. The goal of this study was to better define the conditions for use and their specificity. We found that L-012 coupled with depolymerized orthovanadate, a relatively impermeable tyrosine phosphatase inhibitor, yielded a highly sensitive approach to detect extracellular O₂^●-. In O₂^●- producing HEK-NOX5 cells, orthovanadate increased L-012 luminescence 100-fo

SUBMITTER: Haigh S 

PROVIDER: S-EPMC10525458 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature