Desmin Reorganization by Stimuli Inducing Oxidative Stress and Electrophiles: Role of Its Single Cysteine Residue.

ABSTRACT: The type III intermediate filament proteins vimentin and GFAP are modulated by oxidants and electrophiles, mainly through perturbation of their single cysteine residues. Desmin, the type III intermediate filament protein specific to muscle cells, is critical for muscle homeostasis, playing a key role in sarcomere organization and mitochondrial function. Here, we have studied the impact of oxidants and cysteine-reactive agents on desmin behavior. Our results show that several reactive species and drugs induce covalent modifications of desmin in vitro, of which its single cysteine residue, C333, is an important target. Moreover, stimuli eliciting oxidative stress or lipoxidation, including H₂O₂, 15-deoxy-prostaglandin J₂, and CoCl₂-elicited chemica

SUBMITTER: Moneo-Corcuera D 

PROVIDER: S-EPMC10525603 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature