Lower levels of FOXP3 are associated with prolonged inflammatory responses in kidney transplant recipients.

ABSTRACT:

Background

Immunosuppressive treatment of kidney transplant recipients is mainly aimed at pro-inflammatory T effector cells, yet they also target the immunosuppressive T regulatory cells. Here, we test the hypothesis that low levels of the master gene regulator of T regulatory cells, forkhead box P3 (FOXP3) splice variants, are associated with prolonged inflammatory responses to stimuli.

Methods

From blood samples obtained the first - and 29^th day post-transplant, we extracted peripheral blood mononuclear cells and measured mRNA levels of Total FOXP3, pre-mature RNA FOXP3 (pre-mRNA FOXP3), full length FOXP3 (FOXP3fl) and, FOXP3 splice variant excluding exon two (FOXP3d2). We defined the primary outcome as the number of days in which C reactive protein (CRP) was ab

SUBMITTER: Saleh QW 

PROVIDER: S-EPMC10525697 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature