Engineering and Purification of Microcin C7 Variants Resistant to Trypsin and Analysis of Their Biological Activity.

ABSTRACT: Microcin C7 (McC) as a viable form of antimicrobial has gained substantial attention due to its distinctive antimicrobial activity, by targeting aspartyl tRNA synthetase. McC can be a potential solution against pathogenic microbial infections in the postantibiotic era. However, considering that degradation by digestive enzymes can disrupt the function of this peptide in the gastrointestinal tract, in this study, we attempt to design McC variants to overcome several barriers that may affect its stability and biological activity. The mccA gene encoding the McC peptide precursor was mutated and 12 new McC variants with trypsin resistance were found. The Yej⁺rimL^- strain was used as an indicator to determine the minimum inhibitory concentrations (MICs). The results

SUBMITTER: Yang G 

PROVIDER: S-EPMC10525924 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature