An Anthracene Carboxamide-Based Fluorescent Probe for Rapid and Sensitive Detection of Mitochondrial Hypochlorite in Living Cells.

ABSTRACT: Mitochondrial hypochlorite (ClO^-) plays important and often contradictory roles in maintaining the redox balance of mitochondria. Abnormal ClO^- levels can induce mitochondrial inactivation and further cause cell apoptosis. Herein, we have developed an anthracene carboxyimide-based fluorescent probe mito-ACS for imaging mitochondrial ClO^- in living cells. This probe exhibits some distinctive features as excellent resistance to photobleaching, high selectivity and sensitivity, as well as good water solubility. Mito-ACS showed a noticeable fluorescence response toward ClO^- with a fast response (within 6 s) and a low detection limit (23 nM). Moreover, the introduction of triphenylphosphonium makes the probe soluble in water and selectiv

SUBMITTER: Liu X 

PROVIDER: S-EPMC10526414 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature