Peroxisomal NAD(H) Homeostasis in the Yeast <i>Debaryomyces hansenii</i> Depends on Two Redox Shuttles and the NAD⁺ Carrier, Pmp47.

ABSTRACT: Debaryomyces hansenii is considered an unconventional yeast with a strong biotechnological potential, which can produce and store high amounts of lipids. However, relatively little is known about its lipid metabolism, and genetic tools for this yeast have been limited. The aim of this study was to explore the fatty acid β-oxidation pathway in D. hansenii. To this end, we employed recently developed methods to generate multiple gene deletions and tag open reading frames with GFP in their chromosomal context in this yeast. We found that, similar as in other yeasts, the β-oxidation of fatty acids in D. hansenii was restricted to peroxisomes. We report a series of experiments in D. hansenii and the well-studied yeast Saccharomyces cerevisiae that show that th

SUBMITTER: Turkolmez S 

PROVIDER: S-EPMC10526880 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature