The Impact of Ca²⁺ on Intracellular Distribution of Hemoglobin in Human Erythrocytes.

ABSTRACT: The membrane-bound hemoglobin (Hb) fraction impacts red blood cell (RBC) rheology and metabolism. Therefore, Hb-RBC membrane interactions are precisely controlled. For instance, the signaling function of membrane-bound deoxy-Hb and the structure of the docking sites in the cytosolic domain of the anion exchanger 1 (AE-1) protein are well documented; however, much less is known about the interaction of Hb variants with the erythrocyte's membrane. Here, we identified factors other than O₂ availability that control Hb abundance in the membrane-bound fraction and the possible variant-specific binding selectivity of Hb to the membrane. We show that depletion of extracellular Ca²⁺ by chelators, or its omission from the extracellular medium, leads to membrane-bound Hb releas

SUBMITTER: Livshits L 

PROVIDER: S-EPMC10526966 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature