SARS-CoV-2 niches in human placenta revealed by spatial transcriptomics.

ABSTRACT:

Background

Functional placental niches are presumed to spatially separate maternal-fetal antigens and restrict the vertical transmission of pathogens. We hypothesized a high-resolution map of placental transcription could provide direct evidence for niche microenvironments with unique functions and transcription profiles.

Methods

We utilized Visium Spatial Transcriptomics paired with H&E staining to generate 17,927 spatial transcriptomes. By integrating these spatial transcriptomes with 273,944 placental single-cell and single-nuclei transcriptomes, we generated an atlas composed of at least 22 subpopulations in the maternal decidua, fetal chorionic villi, and chorioamniotic membranes.

Findings

Comparisons of placentae from uninfected healthy controls (n = 4) with COV

SUBMITTER: Barrozo ER 

PROVIDER: S-EPMC10527005 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature